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斯通改良洛克氏液/斯通改良洛克氏溶液
Stone's Modification of Locke's Solution
產品編號: MD591
產品名稱: 斯通改良洛克氏液/斯通改良洛克氏溶液
英文名稱: Stone's Modification of Locke's Solution
生產廠家: 山東拓普生物工程有限公司
產品用途: 用于制備ATCC培養(yǎng)基1671;ATCC Medium: 1671 Blastocystis Egg Medium
瀏覽次數: 141
 
編號 規(guī)格 貯藏條件 保存期限 目錄價 會員價 數量
MD591B250g/瓶常溫3年請登錄請登錄請登錄
產品詳情
培養(yǎng)基配方(每升)
NaCl                         8.0g
CaCl2                        0.2g
KCl                          0.2g
MgCl2·6H2O                 0.01g
Na2HPO4                      2.0g
NaHCO3                       0.4g
KH2PO4                       0.3g

使用說明
稱取本品11.11g于1L蒸餾水中,121℃高壓滅菌15分鐘或115℃滅菌30分鐘,冷卻,按文件規(guī)定的方法制備ATCC培養(yǎng)基1671.
本品有不溶物,分裝時需攪拌使用。

特殊說明
ATCC Medium: 1671 Blastocystis Egg Medium


Whole emulsified egg Stone’s Modification of Locke’s Solution (see below) 783 217 mlml
Use only fresh fertile eggs (one source for such eggs is B&E eggs in York Spring Pa. Ship at Day 0)


Filter through gauze into a filtration flask.


Place under vacuum to draw out air bubbles.


Dispense in 4.0 ml amounts to a 16 x 125 mm screw-cap tube.


Inspissate (80 °C) at a 30 degree slant .Generally it takes approximately 1 hour to set. Remove when eggs have solidified. Be careful not to overcook.


Allow to cool and add a 4.5 ml overlay of Stone’s Modification of Locke’s Solution.


Cap with rubber stoppers then autoclave for 20 minutes at 121°C.


Remove rubber stoppers after the medium is cooled the aseptically replace with screw-caps.


Stone’s Modification of Locke’s Solution*:


NaCl CaCl2 KCl MgCl2 x 6H2O Na2HPO4 NaHCO3 KH2PO4 Distilled water 8.0 0.2 0.2 0.01 2.0 0.4 0.3 ggggggg
1000.0 ml
*Reference: Am. J. Trop. Med., 15, 681- , 1935.


Note: Solution will precipitate when Na2HPO4 is added. Dispense while stirring to evenly distribute salts.


Protistology will add 25% (v/v) heat-inactivated (56C for 30 minutes) horse serum (~1.5ml) to each tube just before use (before placing the tubes in the anaerobic jar 48 hours prior to inoculation with Blastocystis).

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